In 2011, the CBGP opted to set up a metabolomics/mass spectrometry facility. The platform was established and fully productive in spring 2012. The metabolomics laboratory contains equipment for the extraction, pre-purification, and derivatization of small molecules, a triple quadrupole mass spectrometer linked to a gas chromatograph (GC-QqQ-MS/MS), and a hybrid mass spectrometer (ESI-Qq-TOF-MS/MS) that can optionally be coupled to an ultra high performance liquid chromatography (UHPLC) or a nanoLC system. With its high mass accuracy and resolution this set-up facilitates advanced metabolite and small molecule detection.
The metabolomics unit offers metabolomics service to the members of the CBGP at cost price. In addition, the expertise and technology of the facility is made available to external groups on the basis of contracted research projects or scientific collaborations. The service covers absolute quantification of target compounds, such as plant hormones, amino acids, carbohydrates, and their derivatives. Moreover, it offers targeted metabolomics approaches. Following data acquisition and processing, fully evaluated data sets are provided. Additional statistical analysis and custom solutions are also available at an extra charge.
For any kind of inquiry or further questions feel free to contact:
Dr. Stephan POLLMANN, Head of Service [german, english, spanish]
Interested readers can find some more information on Metabolomics in our following publications:
1. Carrasco Loba, V., Pollmann, S. (2017) Highly sensitive salicylic acid quantification in milligram amounts of plant tissue. Methods Mol. Biol. 1497, 221-229.
2. Carrasco Loba, V., Pérez Alonso, M.M., Pollmann, S. (2017) Monitoring of crosstalk between jasmonate and auxin in the framework of plant stress responses of roots. Methods Mol. Biol. 1569, 175-185.
3. Pérez Alonso, M.M., Carrasco Loba, V., Medina, J., Vicente-Carbajosa, J., Pollmann, S. (2018) When transcriptomics and metabolomics work hand in hand: a case study characterizing plant CDF transcription factors. High-Throughput 2, 7.
4. Pérez Alonso, M.M., Carrasco Loba V., Pollmann, S. (2018). Advances in Plant Metabolomics. In: Annual Plant Reviews online, J.A. Roberts (Ed.). doi:10.1002/9781119312994.apr0660.
18. Salinas-Grenet, H. et al. (2018) Int. J. Mol. Sci. 19, 2480.
17. Ramos, P. et al. (2018) Fungal Ecol. 34, 76-82.
16. Abbas, M. et al. (2018) Proc. Natl. Acad. Sci. USA 115, 6864-6869.
15. Mélida, H. et al. (2018) Plant J. 93, 34-49.
14. Sopeña-Torres, S. (2018) New Phytol. 218, 661-680.
13. Corrales, A.R. et al. (2017) Plant Cell Environ. 40, 748-764.
12. Lehmann, T. et al. (2017) Front. Plant Sci. 8, 36.
11. Cubells‐Baeza, N. et al. (2017) Plant Mol. Biol. 94, 33-44.
10. Renau-Morata, B. et al. (2017) Front. Plant Sci. 8, 660.
9. Križnik, M. et al. (2017) Front. Plant Sci. 8, 2192.
8. Garrido-Arandia, M. et al. (2016) Sci. Rep. 6, 33468.
7. de Marchi, R. et al. (2016) Sci. Rep. 6, 26020.
6. Silva-Navas, J. et al. (2016) Plant Cell 28, 1372–1387.
5. Wilcoxen, J. et al. (2016) J. Am. Chem. Soc. 138, 7468-7471.
4. Król, P. et al. (2015) J. Plant Physiol. 179, 121-132.
3. Corrales, A.R. et al. (2014) J. Exp. Bot. 65, 995-1012.
2. Sánchez-Parra, B. et al. (2014) Plants 3, 324-347.
1. Sanz, L. et al. (2014) Plant Physiol. 166, 1972-1984.